application of random amplified polymorphic DNA for sandfly species identification

We have applied the recently developed Random Amplified Polymorphic DNA (RAPD) method to produce species-specific, DNA profiles for two sympatric, Venezuelan sandfly species, thought to be the vectors responsible for recent outbreaks of cutaneous and mucocutaneous leishmaniasis in the Andean State o...

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Bibliographic Details
Published inMedical and veterinary entomology Vol. 7; no. 3; pp. 203 - 207
Main Authors Adamson, R.E, Ward, R.D, Feliciangeli, M.D, Maingon, R
Format Journal Article
LanguageEnglish
Published England 01.07.1993
Subjects
Animals
Base Sequence
DNA
DNA - genetics
Female
identification
insect pests
Insect Vectors - classification
Insect Vectors - genetics
Leishmaniasis - parasitology
Lutzomyia
lutzomyia spinicrassa
lutzomyia youngi
Male
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Psychodidae - classification
Psychodidae - genetics
Venezuela
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Summary:We have applied the recently developed Random Amplified Polymorphic DNA (RAPD) method to produce species-specific, DNA profiles for two sympatric, Venezuelan sandfly species, thought to be the vectors responsible for recent outbreaks of cutaneous and mucocutaneous leishmaniasis in the Andean State of Tachira. Moreover, within the profile, it was possible to identify a diagnostic DNA band for Lu. youngi of 0.32 kb. Results showed that the size of this diagnostic DNA band remained constant and did not vary with sex or geographical distribution.
ISSN:0269-283X
1365-2915
DOI:10.1111/j.1365-2915.1993.tb00677.x