A new approach for bone marrow-derived stem cells intrapancreatic autotransplantation in diabetic rats

• Published in: Microsurgery Vol. 26; no. 7; pp. 539 - 542
• Main Authors: Álvarez, Silvia Salom, Jiménez, Luis Miguel, Murillo, Ana Zugasti, Gómez, Ignacio García, Ligero, José Manuel, Gómez-Pineda, Alfonso, Rollán-Landeras, Eduardo, Cuevas, Pedro, Jara-Albarrán, Antonino
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 2006; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Bone Marrow Cells; Diabetes Mellitus, Experimental - surgery; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Medical sciences; Pancreas - surgery; Rats; Rats, Wistar; Stem Cell Transplantation - methods; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Endocrinopathy; Rat; Stem cell; Diabetes mellitus; Rodentia; Transplantation; Autotransplantation; Vertebrata; Mammalia; Treatment; Animal; Surgery; Microsurgery; Bone marrow; Graft; Intrapancreatic; Pancreas
• ISSN: 0738-1085; 1098-2752
• DOI: 10.1002/micr.20283

Purpose: A new technique for stem cells intrapancreatic autotransplantation in rats. Basic Procedures: Section of a femoral diaphysis and aspirations of the bone marrow in both femoral segments were performed. A Kirschner needle was placed into the femur. Cells were isolated in Ficoll gradients and preserved at 4–6°C. The second day, cells were injected, via aortic celiac trunk, into the pancreas of the same rat. Results: Femoral surgery were well tolerated. Intrapancreatic homing of the injected cells was suggested with methylene blue injection that stained the pancreas, and proved by labeled cells in pancreas sections. Cell counts after Ficoll isolation were 1 × 106 ± 2 × 105 ES. Conclusions: A technique is described for stem cell autotransplantation in rats. First we obtain autologous bone marrow‐derived stem cells. Second, we inject the cells in the pancreas of the donor rat. This approach can be applied to experimental diabetes and other pancreatic processes. © 2006 Wiley‐Liss, Inc. Microsurgery, 2006.