Simultaneous determination of paeoniflorin from total glucosides of paeony in Sprague-Dawley rats and spontaneously hypertensive rats by high-performance liquid chromatography-tandem mass spectrometry: in vivo and in vitro studies

• Published in: Biomedical chromatography Vol. 30; no. 11; pp. 1766 - 1771
• Main Authors: Xiong, Shan, Wang, Yuyun
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.11.2016
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - metabolism; Drugs, Chinese Herbal - pharmacokinetics; Glucosides - blood; Glucosides - chemistry; Glucosides - metabolism; Glucosides - pharmacokinetics; LC-MS/MS; Limit of Detection; Male; Microsomes, Liver - metabolism; Monoterpenes - blood; Monoterpenes - chemistry; Monoterpenes - metabolism; Monoterpenes - pharmacokinetics; Paeonia - chemistry; Paeoniflorin; pharmacokinetics; Rats, Inbred SHR; Rats, Sprague-Dawley; spontaneously hypertensive rats; Tandem Mass Spectrometry - methods; total glucosides of paeony; pharmacokinetics; LC-MS/MS; Paeoniflorin; total glucosides of paeony; spontaneously hypertensive rats
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.3751

Paeoniflorin is a well‐known monoterpene glucoside in the herbal drug that exhibits a number of biological activities. The pharmacokinetic characteristics of paeoniflorin from total glucosides of paeony in spontaneously hypertensive rats (SHR) are still unclear. It is essential to investigate the in vivo and in vitro pharmacokinetic differences of paeoniflorin from total glucosides of paeony in Sprague–Dawley (SD) and SHR. The in vivo pharmacokinetic data were analyzed using DAS 2.0 software and the in vitro metabolic characteristics were measured using rat hepatic microsomes. The concentration of paeoniflorin in biological samples was determined using high‐performance liquid chromatography–electrospray ionization tandem mass spectrometry method, which showed good precision and stability. The plasma concentration–time profiles of paeoniflorin following oral administration of total glucosides of paeony showed a single peak and there were significant differences in the mean values of AUC(0–t), AUC(0–∞), CLz/F and Tmax between SD and SHR (p < 0.05). The metabolic rate of paeoniflorin from total glucosides of paeony was slower in SHR than in SD rats (p < 0.05). The results might be useful in further applications of paeoniflorin and total glucosides of paeony. Copyright © 2016 John Wiley & Sons, Ltd.