Cytokines as diagnostic markers of pulpal inflammation
Aim To measure and compare the levels of the cytokines IL‐2, IL‐6, IL‐8, IL‐10, TNF‐α and IFN‐γ in pulpal blood from irreversible pulpitis, asymptomatic caries exposure and normal pulps. Methodology Blood was obtained from pulp exposure sites using cotton pellets. Twenty‐five samples were obtained f...
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Published in | International endodontic journal Vol. 46; no. 6; pp. 573 - 580 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.06.2013
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Subjects | |
Online Access | Get full text |
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Summary: | Aim
To measure and compare the levels of the cytokines IL‐2, IL‐6, IL‐8, IL‐10, TNF‐α and IFN‐γ in pulpal blood from irreversible pulpitis, asymptomatic caries exposure and normal pulps.
Methodology
Blood was obtained from pulp exposure sites using cotton pellets. Twenty‐five samples were obtained from normal teeth, 40 from asymptomatic caries‐exposed pulps and 43 from irreversible pulpitis teeth. Cytokine levels were determined by high‐sensitivity ELISA. Data were statistically analysed using Kruskal–Wallis and Mann–Whitney U‐tests.
Results
Significantly higher levels (P < 0.05) of IL‐6, IL‐8, IL‐10, TNF‐α and IFN‐γ were detected in caries‐exposed pulps and irreversible pulpitis as compared to normal teeth. IL‐2 and IL‐10 levels were significantly higher (P < 0.05) in caries‐exposed pulps as compared to irreversible pulpitis, whilst IL‐8 was significantly higher (P < 0.001) in irreversible pulpitis as compared to caries‐exposed teeth. Most interestingly, IL‐6/IL‐10 and IL‐8/IL‐10 ratios were significantly higher (P < 0.001) in irreversible pulpitis compared with both caries‐exposed and normal teeth.
Conclusion
Levels of IL‐8 and the ratios of IL‐6/IL‐10 and IL‐8/IL‐10 have the potential to be indicators of pulpal inflammation in caries exposure cases. Cytokine estimation in pulpal blood may help in the diagnosis of pulpal inflammation. |
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Bibliography: | ArticleID:IEJ12030 istex:0F670D41C554DF0A74625C63301653BA5CA3AA60 ark:/67375/WNG-Z6R9579F-4 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0143-2885 1365-2591 |
DOI: | 10.1111/iej.12030 |