Age-dependent plasticity in endocannabinoid modulation of pain processing through postnatal development

• Published in: Pain (Amsterdam) Vol. 158; no. 11; pp. 2222 - 2232
• Main Authors: Kwok, Charlie H-T., Devonshire, Ian M., Imraish, Amer, Greenspon, Charles M., Lockwood, Stevie, Fielden, Catherine, Cooper, Andrew, Woodhams, Stephen, Sarmad, Sarir, Ortori, Catherine A., Barrett, David A., Kendall, David, Bennett, Andrew J., Chapman, Victoria, Hathway, Gareth J.
• Format: Journal Article
• Language: English
• Published: United States Wolters Kluwer 01.11.2017
• Subjects: Age Factors; Aging - physiology; Animals; Animals, Newborn; Arachidonic Acids - therapeutic use; Disease Models, Animal; Endocannabinoids - genetics; Endocannabinoids - metabolism; Endocannabinoids - therapeutic use; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Developmental - physiology; Medulla Oblongata - drug effects; Medulla Oblongata - growth & development; Microinjections; Neuronal Plasticity - physiology; Opioid Peptides - metabolism; Opioid Peptides - pharmacology; Pain - drug therapy; Pain - metabolism; Pain Measurement; Periaqueductal Gray - drug effects; Periaqueductal Gray - growth & development; Phospholipase D - genetics; Phospholipase D - metabolism; Polyunsaturated Alkamides - therapeutic use; Rats; Receptors, Cannabinoid - genetics; Receptors, Cannabinoid - metabolism; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Research Paper; RNA, Messenger - metabolism
• ISSN: 0304-3959; 1872-6623
• DOI: 10.1097/j.pain.0000000000001027

Significant age- and experience-dependent remodelling of spinal and supraspinal neural networks occur, resulting in altered pain responses in early life. In adults, endogenous opioid peptide and endocannabinoid (ECs) pain control systems exist which modify pain responses, but the role they play in acute responses to pain and postnatal neurodevelopment is unknown. Here, we have studied the changing role of the ECs in the brainstem nuclei essential for the control of nociception from birth to adulthood in both rats and humans. Using in vivo electrophysiology, we show that substantial functional changes occur in the effect of microinjection of ECs receptor agonists and antagonists in the periaqueductal grey (PAG) and rostroventral medulla (RVM), both of which play central roles in the supraspinal control of pain and the maintenance of chronic pain states in adulthood. We show that in immature PAG and RVM, the orphan receptor, GPR55, is able to mediate profound analgesia which is absent in adults. We show that tissue levels of endocannabinoid neurotransmitters, anandamide and 2-arachidonoylglycerol, within the PAG and RVM are developmentally regulated (using mass spectrometry). The expression patterns and levels of ECs enzymes and receptors were assessed using quantitative PCR and immunohistochemistry. In human brainstem, we show age-related alterations in the expression of key enzymes and receptors involved in ECs function using PCR and in situ hybridisation. These data reveal that significant changes on ECs that to this point have been unknown and which shed new light into the complex neurochemical changes that permit normal, mature responses to pain.