Microsystem for the single molecule analysis of membrane transport proteins

Micro-chamber arrays enable highly sensitive and quantitative bioassays at the single-molecule level. Accordingly, they are widely used for ultra-sensitive biomedical applications, e.g., digital PCR and digital ELISA. However, the versatility of micro-chambers is generally limited to reactions in aq...

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Bibliographic Details
Published inBiochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129330
Main Author Watanabe, Rikiya
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.02.2020
Subjects
aqueous solutions
bioassays
Biomembrane
bioMEMS
enzyme-linked immunosorbent assay
lipid bilayers
Membrane protein
polymerase chain reaction
Single molecule biophysics
transporters
bioMEMS
Membrane protein
Single molecule biophysics
Biomembrane
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Summary:Micro-chamber arrays enable highly sensitive and quantitative bioassays at the single-molecule level. Accordingly, they are widely used for ultra-sensitive biomedical applications, e.g., digital PCR and digital ELISA. However, the versatility of micro-chambers is generally limited to reactions in aqueous solutions, although various functions of membrane proteins are extremely important. To address this issue, microsystems using arrayed micro-sized chambers sealed with lipid bilayers, referred to here as a “biomembrane microsystems”, have been developed by many research groups for the analysis of membrane proteins. In this review, I would like to introduce recent progress on the single molecule analysis of membrane transport proteins using a biomembrane microsystem, and discuss the future prospects for its use in analytical and pharmacological applications.
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ISSN:0304-4165
1872-8006
1872-8006
DOI:10.1016/j.bbagen.2019.03.016