Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury

Non-alcoholic liver injury (NLI) is a common disease worldwide. Since free radical damage in the liver is a crucial initiator leading to diseases, scavenging excess free radicals has become an essential therapeutic strategy. To enhance the antioxidant capacity of histidine, we synthesized a protonat...

Full description

Saved in:
Bibliographic Details
Published inAntioxidants Vol. 10; no. 10; p. 1529
Main Authors Zhao, Zizhen, Fu, Chen, Zhang, Yuping, Fu, Ailing
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 27.09.2021
MDPI
Subjects
Animal models
Animals
Antioxidants
Biochemistry
Carbon tetrachloride
Cell culture
Cell viability
Diet
Experiments
Fatty acids
Fatty liver
Fibrosis
Fourier transforms
Free radicals
H-bihistidine
Hepatocytes
High fat diet
Histidine
Hydrogen peroxide
Lipids
Liver
liver fibrosis
NMR
nonalcoholic liver injury
Nuclear magnetic resonance
oxidation injury
Oxidative stress
Spectrum analysis
Beijing China
United States > US
China
Germany
Online AccessGet full text

Cover

More Information
Summary:Non-alcoholic liver injury (NLI) is a common disease worldwide. Since free radical damage in the liver is a crucial initiator leading to diseases, scavenging excess free radicals has become an essential therapeutic strategy. To enhance the antioxidant capacity of histidine, we synthesized a protonated dimeric histidine, H-bihistidine, and investigated its anti-free radical potential in several free-radical-induced NLI. Results showed that H-bihistidine could strongly scavenge free radicals caused by H2O2, fatty acid, and CCl4, respectively, and recover cell viability in cultured hepatocytes. In the animal model of nonalcoholic fatty liver injury caused by high-fat diet, H-bihistidine reduced the contents of transaminases and lipids in serum, eliminated the liver’s fat accumulation, and decreased the oxidative damage. Moreover, H-bihistidine could rescue CCl4-induced liver injury and recover energy supply through scavenging free radicals. Moreover, liver fibrosis prepared by high-fat diet and CCl4 administration was significantly alleviated after H-bihistidine treatment. This study suggests a novel nonenzymatic free radical scavenger against NLI and, potentially, other free-radical-induced diseases.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox10101529