High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family

Cucumber mosaic virus (CMV; Cucumovirus, Bromoviridae) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variant...

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Published inPlants (Basel) Vol. 11; no. 13; p. 1665
Main Authors Mrkvová, Michaela, Hančinský, Richard, Predajňa, Lukáš, Alaxin, Peter, Achs, Adam, Tomašechová, Jana, Šoltys, Katarína, Mihálik, Daniel, Olmos, Antonio, Ruiz-García, Ana Belén, Glasa, Miroslav
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 23.06.2022
MDPI
Subjects
Amino acids
CMV
Cucumbers
cucumovirus
Divergence
Epidemiology
Flowers & plants
genetic diversity
Genetic variability
genome
Genomes
high-throughput sequencing
Host plants
Host range
Infections
Next-generation sequencing
Papaver
Papaveraceae
Phylogeny
Plant virus diseases
Poppies
Proteins
Ribonucleic acid
RNA
RNA polymerase
Satellite RNA
Viruses
Slovakia
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Summary:Cucumber mosaic virus (CMV; Cucumovirus, Bromoviridae) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variants originated from the Papaveraceae species (oilseed poppy, common poppy and great celandine), previously poorly described as CMV natural hosts. Based on a BLAST search and phylogenetic analysis, the Slovak CMV isolates can be divided into two genetically different Groups, Ia and II, respectively. The SL50V variant, characterized by a divergent RNA2 sequence, potentially represents a reassortant variant. In four samples (T101, SL50V, CP2, MVU2-21), the presence of satellite CMV RNA was identified along with CMV. Although mechanically transmitted to experimental cucumber plants, the role of satellite RNA in the symptomatology observed could not be established due to a complex infection of original hosts with different viruses.
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These authors contributed equally to this work.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11131665