Identification of Histamine Receptors and Reduction of Squalene Levels by an Antihistamine in Sebocytes
Overproduction of sebum, especially during adolescence, is causally related to acne and inflammation. As a way to reduce sebum and its interference with the process of follicular keratinization in the pilosebaceous unit leading to inflammatory acne lesions, antihistamines were investigated for their...
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Published in | Journal of investigative dermatology Vol. 128; no. 5; pp. 1280 - 1285 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Danvers, MA
Elsevier Inc
01.05.2008
Nature Publishing Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | Overproduction of sebum, especially during adolescence, is causally related to acne and inflammation. As a way to reduce sebum and its interference with the process of follicular keratinization in the pilosebaceous unit leading to inflammatory acne lesions, antihistamines were investigated for their effect on sebocytes, the major cell of the sebaceous gland responsible for producing sebum. Reverse transcriptase-PCR analysis and immunofluorescence of an immortalized sebocyte cell line (SZ95) revealed the presence of histamine-1 receptor (H-1 receptor), and thus indicated that histamines and, conversely, antihistamines could potentially modulate sebocyte function directly. When sebocytes were incubated with an H-1 receptor antagonist, diphenhydramine (DPH), at non-cytotoxic doses, a significant decrease in squalene levels, a biomarker for sebum, was observed. As determined by high-performance liquid chromatography, untreated sebocytes contained 6.27 (±0.73)nmol squalene per 106 cells, whereas for DPH-treated cells, the levels were 2.37 (±0.24) and 2.03 (±0.97)nmol squalene per 106 cells at 50 and 100μM, respectively. These data were further substantiated by the identification of histamine receptors in human sebaceous glands. In conclusion, our data show the presence of histamine receptors on sebocytes, demonstrate how an antagonist to these receptors modulated cellular function, and may indicate a new paradigm for acne therapy involving an H-1 receptor-mediated pathway. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-202X 1523-1747 |
DOI: | 10.1038/sj.jid.5701160 |