The Effect of Rhodamine B on the Cerebellum and Brainstem Tissue of Rattus Norvegicus

Background Rhodamine B is a component of xenobiotic substance metabolized by cytochrome P450 in the body to produce free radicals, which affects the activity of Superoxidase Dismutase (SOD), thereby, leading to oxidative stress, injury, increase in cell apoptosis and brainstem. This study aims to de...

Full description

Saved in:
Bibliographic Details
Published inJournal of public health research Vol. 9; no. 2
Main Authors Sulistina, Dewi Ratna, Martini, Santi
Format Journal Article
LanguageEnglish
Published Pavia PAGEPress Publications 03.07.2020
PAGEPress Publications, Pavia, Italy
SAGE Publishing
Subjects
Apoptosis
BAX, BCL-2
Brain
Brain research
Cerebellum and Brainstem tissue
Cytochrome
Epidemics
Food contamination & poisoning
Free radicals
Medical research
Oxidative stress
Public health
Rhodamine B
Spinal cord
Tissues
Online AccessGet full text

Cover

More Information
Summary:Background Rhodamine B is a component of xenobiotic substance metabolized by cytochrome P450 in the body to produce free radicals, which affects the activity of Superoxidase Dismutase (SOD), thereby, leading to oxidative stress, injury, increase in cell apoptosis and brainstem. This study aims to determine the effect of Rhodamine B on BAX and BCL-2 in the cerebellum and brainstem tissue of Rattus norvegicus. Design and methods The True Experimental Design was used to carry out a post-test examination on the control group of twenty-eight Wistar female Rattus norvegicus mice between the ages of 10-12 weeks. Then, samples were categorized into 4 groups in body weight doses of 4.5 mg/200g, 9 mg/200g, and 18 mg/200g. They were administrated with Rhodamine B personde for 36 days. Results The results showed that Rhodamine B had a direct and indirect effect on BAX and BCL-2 expressions, respectively, in the cerebellum tissue and wistar strain of Rattus norvegicus. In addition, the positive path coefficient of BAX expression has a positive effect on BCL-2. This means that an increase in BAX has a direct impact on decreasing BCL-2 expression in cerebellum tissue and brainstem of Rattus norvegicus wistar strain along with an increased dose of Rhodamine B. Conclusions In conclusion, Rhodamine B tends to increase BAX expression which directly decreases BCL-2 in Cerebellum tissue and Brainstem in Rattus norvegicus along with increasing doses.
Bibliography:Conference presentation: Part of this paper was presented at the 4rd International Symposium of Public Health, from Oct 29–31, 2019, at Griffith University, Gold Coast, Brisbane, Queensland, Australia.
Clinical trials: This research has been approved by the Research Ethics Committee of The Faculty of Medicine University of Brawijaya, Malang, Indonesia
Contributions: The authors contributed equally.
Conflict of interest: The authors declare no potential conflict of interest.
ISSN:2279-9036
2279-9028
2279-9036
DOI:10.4081/jphr.2020.1812