Degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures

• Published in: The Science of the total environment Vol. 435-436; pp. 402 - 410
• Main Authors: Pinto, A.P., Serrano, C., Pires, T., Mestrinho, E., Dias, L., Teixeira, D. Martins, Caldeira, A.T.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2012
• Subjects: Aniline Compounds - metabolism; Aspergillus oryzae; Biodegradation; Biodegradation, Environmental; chronic exposure; culture media; Difenoconazole; Dioxolanes - metabolism; Fungi; Fungi - metabolism; Fusarium oxysporum; Lentinula edodes; Pendimethalin; Penicillium brevicompactum; Pesticide Residues - metabolism; Soil Pollutants - metabolism; soil pollution; Terbuthylazine; Triazines - metabolism; Triazoles - metabolism; Water Pollutants, Chemical - metabolism; water pollution; water resources; Europe; Biodegradation; Fungi; Difenoconazole; Terbuthylazine; Pendimethalin
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2012.07.027

Contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating European water resources. The aim of this work was to evaluate the ability of the fungi Fusarium oxysporum, Aspergillus oryzae, Lentinula edodes, Penicillium brevicompactum and Lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. These pesticides are common soil and water contaminants and terbuthylazine is considered the most persistent triazine herbicide in surface environments. P. brevicompactum and L. saksenae were achieved by enrichment, isolation and screening of fungi capable to metabolize the pesticides studied. The isolates were obtained from two pesticide-primed materials (soil and biomixture). Despite the relatively high persistence of terbuthylazine, the results obtained in this work showed that the fungi species studied have a high capability of biotransformation of this xenobiotic, comparatively the results obtained in other similar studies. The highest removal percentage of terbuthylazine from liquid medium was achieved with A. oryzae (~80%), although the major biodegradation has been reached with P. brevicompactum. The higher ability of P. brevicompactum to metabolize terbuthylazine was presumably acquired through chronic exposure to contamination with the herbicide. L. saksenae could remove 99.5% of the available pendimethalin in batch liquid cultures. L. edodes proved to be a fungus with a high potential for biodegradation of pesticides, especially difenoconazole and pendimethalin. Furthermore, the metabolite desethyl-terbuthylazine was detected in L. edodes liquid culture medium, indicating terbuthylazine biodegradation by this fungus. The fungi strains investigated could prove to be valuable as active pesticide-degrading microorganisms, increasing the efficiency of biopurification systems containing wastewaters contaminated with the xenobiotics studied or compounds with similar intrinsic characteristics. ► Biodegradation of terbuthylazine, difenoconazole and pendimethalin was evaluated. ► Fungi capable to degrade the pesticides were isolated from a soil and a biomixture. ► All the fungi tested were very efficient in the biodegradation of the pesticides. ► L. saksenae (isolated fungi) could remove 99.5% of the pendimethalin. ► The biosorption of pesticides proved to be a process dependent on the fungi species.