Partial characterization and anticoagulant activity of sulfated galactan from the green seaweed Halimeda opuntia

The number of deaths associated with cardiovascular diseases (CVD) increases every year, leading to an intense search for new compounds that may be employed as anticoagulants. One of the classes of bioprospected molecules comprises sulfated polysaccharides (SP) from seaweed, as heparin displays many...

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Published inAnais da Academia Brasileira de Ciências Vol. 95; no. 2; p. e20211002
Main Authors Costa, Bianca B, Gianelli, Jessica L D, Moreira, Thamyris A, Soares, Angelica R, Glauser, Bianca F, Mourão, Paulo A S, D Neto, Chaquip, Barros, Cinthia M, Cinelli, Leonardo P
Format Journal Article
LanguageEnglish
Published Brazil Academia Brasileira de Ciências 01.01.2023
Subjects
Anticoagulants
Blood clotting
Chlorophyta
Factor IIa
Galactans
Heparin-like
Marine algae
MULTIDISCIPLINARY SCIENCES
Opuntia
Polysaccharides
Seaweed
Sulfated polysaccharides
Sulfates
Blood clotting
Sulfated polysaccharides
Factor IIa
Marine algae
Heparin-like
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Summary:The number of deaths associated with cardiovascular diseases (CVD) increases every year, leading to an intense search for new compounds that may be employed as anticoagulants. One of the classes of bioprospected molecules comprises sulfated polysaccharides (SP) from seaweed, as heparin displays many adverse effects associated with its use. The present study aimed to characterize and evaluate the anticoagulant potential of SP extracted from the green algae Halimeda opuntia. Four PS-rich fractions, F23, F44, F60 and F75, were obtained by proteolytic digestion in papain followed by ethanol precipitation. The presence of SP was confirmed by agarose gel electrophoresis, revealing different populations in each fraction. The F44 fraction is noteworthy compared to the other fractions, presenting a 5% yield compared to the initial algae weight and anticoagulant activity revealed by the activated partial thromboplastin time (APTT) assay (intrinsic/common coagulation pathway). Surprisingly, F44 purification (SP peak P1F44) resulted in prothrombin time (PT) activity (extrinsic coagulation pathway) at a 160 µg/mL, in addition to enhanced APTT activity. The P1F44 anticoagulant activity mechanism was shown to be dependent on two coagulations factors, IIa and Xa, more potent via IIa. Future assessments will be performed to assess this fraction in the medical clinic.
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ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202320211002