Distribution of Saccharide Residues on Membrane Fragments from a Myeloma-Cell Homogenate: Its Implications for Membrane Biogenesis

Ferritin conjugates of two plant agglutinins, concanavalin A and ricin, have been used as specific electron microscopic stains for covalently-bound saccharide residues on membrane fragments from a myeloma-cell homogenate. The results indicate that different saccharide residues are uniformly localize...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 69; no. 10; pp. 2945 - 2949
Main Authors Hirano, Hiroshi, Parkhouse, Brenda, Nicolson, Garth L., Lennox, Edwin S., Singer, S. J.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.10.1972
National Acad Sciences
Subjects
Agglutinins
Animals
Biological Sciences: Cell Biology
Cell Line
Cell Membrane - metabolism
Cell membranes
Centrifugation
Concanavalin A
Erythrocyte membrane
Eukaryotic cells
Ferritins
Glycoproteins
Mice
Mice, Inbred BALB C
Microscopy, Electron
Multiple Myeloma - pathology
Myeloma Proteins - metabolism
Oligosaccharides
Oligosaccharides - metabolism
P branes
Plasmacytoma - metabolism
Protein Binding
Ricin
Rough endoplasmic reticulum
Staining and Labeling
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Summary:Ferritin conjugates of two plant agglutinins, concanavalin A and ricin, have been used as specific electron microscopic stains for covalently-bound saccharide residues on membrane fragments from a myeloma-cell homogenate. The results indicate that different saccharide residues are uniformly localized to a single surface of each membrane fragment. In particular, the ferritin-concanavalin A conjugate binds exclusively to the cisternal side of membrane fragments of the rough endoplasmic reticulum. If it is postulated that the biogenesis of eukaryotic plasma membranes involves an assembly-line process from process from precursor intracellular membranes, these observed asymmetric distributions of saccharides on cell membranes can be explained.
Bibliography:To whom reprint requests should be addressed.
Present address: Department of Molecular Biology, University of California at Berkeley, Berkeley, Calif. 94720.
Present address: Department of Anatomy, Kyorin University Medical School, Shinkawa Mitaka, Tokyo, Japan.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.10.2945