Altered calcium-handling properties of jejunal smooth muscle from the nematode-infected rat

We examined changes in contractility of jejunal longitudinal muscle from rats infected 8 days previously with the enteric parasite Nippostrongylus brasiliensis. In uninfected control rats, carbachol-induced contraction was maximal at 1 microM carbachol. In muscle from infected rats, there was no cha...

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Published inGastroenterology (New York, N.Y. 1943) Vol. 91; no. 6; p. 1462
Main Authors Fox-Robichaud, A E, Collins, S M
Format Journal Article
LanguageEnglish
Published United States 01.12.1986
Subjects
Animals
Calcium - physiology
Carbachol - pharmacology
In Vitro Techniques
Intestinal Diseases, Parasitic - metabolism
Intestinal Diseases, Parasitic - physiopathology
Jejunum - metabolism
Jejunum - physiopathology
Male
Muscle Contraction - drug effects
Muscle, Smooth - physiopathology
Nematode Infections - metabolism
Nematode Infections - physiopathology
Nippostrongylus
Nitrendipine - pharmacology
Quinuclidinyl Benzilate - metabolism
Radioligand Assay
Rats
Rats, Inbred Strains
Receptors, Muscarinic - metabolism
Serotonin - pharmacology
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Summary:We examined changes in contractility of jejunal longitudinal muscle from rats infected 8 days previously with the enteric parasite Nippostrongylus brasiliensis. In uninfected control rats, carbachol-induced contraction was maximal at 1 microM carbachol. In muscle from infected rats, there was no change in ED50 for carbachol, but contraction induced by 1 microM carbachol was increased greater than 75% over that in control rats. No significant differences were observed in muscarinic receptor binding characteristics in smooth muscle cells from control and infected rats when [3H]QNB was used as radioligand. Contraction induced by 5-hydroxytryptamine was also substantially greater in muscle from infected rats. In control rats, carbachol-induced contraction was largely independent of extracellular Ca2+, whereas in muscle from infected rats, withdrawing extracellular Ca2+ reduced contraction to below control levels. Furthermore, whereas adding nitrendipine in the presence of extracellular Ca2+ had no effect on carbachol-induced contraction in control rats, it significantly inhibited contraction in muscle from infected rats.
ISSN:0016-5085
DOI:10.1016/0016-5085(86)90202-7