Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

• Published in: Anais da Academia Brasileira de Ciências Vol. 88; no. 2; pp. 877 - 890
• Main Authors: Yañez, Agustina, Marquez, Gonzalo J, Morbelli, Marta A
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Academia Brasileira de Ciências 13.05.2016
• Subjects: Dennstaedtia ceae; Dennstaedtiaceae - physiology; Esporos; Microlepia speluncae; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; MULTIDISCIPLINARY SCIENCES; Província Paranaense; Pteridium - physiology; Pteridium arachnoideum; Sporangia; Spores - ultrastructure; ultraestrutura; Esporos; Dennstaedtiaceae; Província Paranaense; ultraestrutura; Paranaense Province; ultrastructure; Dennstaedtia ceae; Microlepia speluncae; Spores; Pteridium arachnoideum
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/0001-3765201620150230

The spore morphology and wall ultrastructure of Microlepia speluncae and Pteridium arachnoideum from the Paranaense Province were analyzed with LM, SEM and TEM and a comparative analysis was carried out. In both species the spores are covered by a three-dimensional network of threads branched and fused, tangentially arranged to the surface, and some free-end threads are also seen. The species were differentiated by morphology and the frequency of threads fusion and the networks distribution on the surface of the spores. In both species the exospore is two-layered in section, both layers are traversed by single or branched channels. The perispore is three-layered in section: the inner layer is adhered to the exospore, the middle layer is formed of a three-dimensional network of threads and the outer layer is discontinuous. The perispore ultrastructure of Microlepia speluncae was interpreted as formed of helical subunits displayed around a central channel. The spore morphology and perispore ultrastructure allow differentiating Microlepia from Pteridium but also to the other Dennstaedtiaceae genera that grow in the Paranaense Province. The results obtained allow establishing relationships that let us recognize different groups and gave a new reference to get a better knowledge of the family.