Fertility of cryopreserved sperm in three bulls with different Robertsonian translocations

• Published in: Animal reproduction science Vol. 86; no. 1; pp. 27 - 36
• Main Authors: Molteni, L., Meggiolaro, D., Macchi, A. De Giovanni, Lorenzi, L. De, Crepaldi, P., Stacchezzini, S., Cremonesi, F., Ferrara, F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2005
• Subjects: acrosome; animal genetics; Animals; bulls; Cattle - genetics; Cattle - physiology; Cattle-male reproduction; Chromosomal translocations; chromosome translocation; Cryopreservation; cumulus oophorus; embryo culture; embryonic mortality; Female; Fertility - genetics; Fertilization in Vitro - standards; Fertilization in Vitro - veterinary; Flow Cytometry - veterinary; in vitro fertilization; karyotyping; Male; male fertility; Male sterility; oocytes; Semen Preservation - methods; Sperm Capacitation - physiology; spermatogenesis; spermatozoa; Spermatozoa - physiology; Translocation, Genetic; viability; Cattle-male reproduction; Male sterility; Chromosomal translocations
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2004.05.024

The fertility of three bulls carrying different Robertsonian translocations (rob(1;29), rob(14;17) and rob(26;29)) was evaluated. Oocytes-cumulus complexes obtained from slaughterhouse-derived ovaries were matured and then fertilised in vitro with frozen/thawed seminal material from the above mentioned subjects, and from control bulls with normal karyotype. An assessment was first made of the concentration, vitality and acrosome integrity of the seminal material to be sure that possible differences in the results of the in vitro fertilisation experiments were not due to seminal material quality. The results of the experiments, evaluated by the percentage of cleaved embryos and blastocysts per cleaved embryo, indicated that the three bulls carrying Robertsonian translocations had similar fertilising power and semen qualitative parameters to the controls. These data suggest that neither gametogenesys impairment nor decreased spermatozoa fertilising capacity is responsible for the reduced fertility in bulls with Robertsonian translocations. What the data do confirm is that the observed in vivo hypofertility for karyologically abnormal bulls is mainly due to early embryonic mortality.