Proteomic identification of tumor- and metastasis-associated galectin-1 in claudin-low breast cancer

• Published in: Biochimica et biophysica acta. General subjects Vol. 1865; no. 2; p. 129784
• Main Authors: Balestrieri, Kassondra, Kew, Kimberly, McDaniel, Moses, Ramez, Mohamed, Pittman, H. Keith, Murray, Gina, Vohra, Nasreen A., Verbanac, Kathryn M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2021
• Subjects: Animals; breast neoplasms; Breast Neoplasms - genetics; Breast Neoplasms - pathology; breasts; Claudin-low breast cancer; Claudins - analysis; Claudins - genetics; Female; Galectin 1 - analysis; Galectin 1 - genetics; Galectin-1; galectins; gene expression regulation; Gene Expression Regulation, Neoplastic; genomics; Humans; Immunohistochemistry; lungs; Mass spectrometry; Metastasis; Mice; Mice, Inbred BALB C; microarray technology; mortality; neoplasm progression; Proteomics; RNA; T11; Galectin-1; Claudin-low breast cancer; Metastasis; T11; Mass spectrometry; Proteomics
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2020.129784

Metastasis and mortality remain high among breast cancer patients with the claudin-low subtype because these tumors are aggressive, chemoresistant, and lack targeted therapies. Our objective was to utilize discovery-based proteomics to identify proteins associated with claudin-low primary and metastatic tumors to gain insight into pathways and mechanisms of tumor progression. We used nano-LC-MS/MS proteomics to analyze orthotopic and metastatic tumors from the syngeneic murine T11 tumor model, which displays gene expression profiles mirroring human claudin-low tumors. Galectin-1 identity, expression and spatial distribution were investigated by biochemical and immunochemical methods and MALDI/IMS. RNA seq data from mouse and human tumors in our study and publicly available microarray data were analyzed for differential galectin-1 expression across breast cancer subtypes. Galectin-1, an N-acetyllactosamine-binding protein, exhibited the highest sequence coverage and high abundance rank order among nano-LC-MS/MS-identified proteins shared by T11 claudin-low tumors but not normal tissue. Label-free quantitation, Western immunoblot and ELISA confirmed galectin-1 identity and significant differential expression. MALDI/IMS spatial mapping and immunohistochemistry detected galectin-1 in T11 metastatic lung foci. Immunohistochemistry of human claudin-low tumors demonstrated intermediate-to-high intensity galectin-1 staining of tumor and stroma. Gene expression analysis of mouse and human tumors found the highest galectin-1 levels in the claudin-low breast cancer subtype. Proteomics and genomics reveal high expression of galectin-1 protein and RNA in primary and metastatic claudin-low breast cancer. This work endorses proteomic approaches in cancer research and supports further investigations of the function and significance of galectin-1 overexpression in claudin-low tumor progression. •Proteomics identify differentially-expressed claudin-low breast cancer biomarkers.•Murine primary and metastatic claudin-low breast cancers express high galectin-1.•Biochemical and immunohistochemical methods validate Galectin-1 proteomics results.•Imaging mass spectrometry maps galectin-1 spatial distribution to lung metastases.•Genomics analysis reveals high galectin-1 in murine and human claudin-low cancer.