Phytochemical Screening, Antioxidant and Antifungal Activities of Aconitum chasmanthum Stapf ex Holmes Wild Rhizome Extracts

• Published in: Antioxidants Vol. 11; no. 6; p. 1052
• Main Authors: Rafiq, Shah, Wagay, Nasir Aziz, Elansary, Hosam O., Malik, Mansoor Ahmad, Bhat, Irshad Ahmad, Kaloo, Zahoor Ahmad, Hadi, Abdul, Alataway, Abed, Dewidar, Ahmed Z., El-Sabrout, Ahmed M., Yessoufou, Kowiyou, Mahmoud, Eman A.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26.05.2022; MDPI
• Subjects: Acetic acid; Aconitum; Antifungal activity; antioxidant assays; Antioxidants; Asthma; Chemicals; Chromatography; Corn; Endangered & extinct species; Endangered species; Ethyl acetate; extraction; Flavonoids; Food poisoning; Free radicals; Hydroxyl radicals; IC50 value; Illnesses; Infections; Mass spectrometry; Medicinal plants; Metabolites; MIC value; Minimum inhibitory concentration; Pesticides; Phenolic compounds; Physiology; phytochemical analysis; Phytochemicals; Plant diseases; Pneumonia; Rhizomes; Scientific imaging; Solvents; Tropical diseases; Mumbai India; India
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox11061052

Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from Kashmir Himalayas, was studied for its antioxidant and antifungal properties. The shade-dried powdered rhizome was extracted sequentially with hexane, ethyl acetate, and methanol. These subsequent fractions were evaluated for total phenolic content (TPC); total flavonoid content (TFC); antioxidant assays, such as 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH); ferric-reducing antioxidant power (FRAP); superoxide radical scavenging (SOR); hydroxyl radical scavenging (OH) and antifungal activity using the poisoned food technique. Highest TPC (5.26 ± 0.01 mg/g) and TFC (2.92 ± 0.04 mg/g) were reported from methanolic extracts. The highest values of radical scavenging activities were also observed in methanolic extracts with IC50 values of 163.71 ± 2.69 μg/mL in DPPH, 173.69 ± 4.91 μg/mL in SOR and 159.64 ± 2.43 μg/mL in OH. The chemical profile of ethyl acetate extract was tested using HR-LCMS. Methanolic extracts also showed a promising inhibition against Aspergillus niger (66.18 ± 1.03), Aspergillus flavus (78.91 ± 1.19) and Penicillium notatum (83.14 ± 0.97) at a 15% culture filtrate concentration with minimum inhibitory concentration (MIC) values of 230 μg/mL, 200 μg/mL and 190 μg/mL, respectively. Overall, the methanolic fractions showed significant biological potential, and its pure isolates might be used to construct a potential new medicinal source.