Retina transduction by rAAV2 after intravitreal injection: comparison between mouse and rat

• Published in: Gene therapy Vol. 26; no. 12; pp. 479 - 490
• Main Authors: Dias, Mariana S, Araujo, Victor G, Vasconcelos, Taliane, Li, Qiuhong, Hauswirth, William W, Linden, Rafael, Petrs-Silva, Hilda
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.12.2019
• Subjects: Adjuvants, Pharmaceutic - administration & dosage; Animals; Clinical trials; Dependovirus - genetics; Diabetic retinopathy; Efficiency; Electroretinography; Gene therapy; Genetic Therapy; Genetic vectors; Genetic Vectors - administration & dosage; Genistein - administration & dosage; Imatinib Mesylate - administration & dosage; Intravitreal Injections; Isoflavones; Mice; Mutation; Oxytetracycline; Phosphorylation; Protein Kinase Inhibitors - administration & dosage; Proteins; Rats; Retina; Retina - virology; Transduction, Genetic; Tyrosine; Vectors (Biology); Canada
• ISSN: 0969-7128; 1476-5462
• DOI: 10.1038/s41434-019-0100-9

Adeno-associated virus vectors (rAAV) are currently the most common vehicle used in clinical trials of retinal gene therapy, usually delivered through subretinal injections to target cells of the outer retina. However, targeting the inner retina requires intravitreal injections, a simple and safe procedure, which is effective for transducing the rodent retina, but still of low efficiency in the eyes of primates. We investigated whether adjuvant pharmacological agents may enhance rAAV transduction of the retinas of mouse and rat after intravitreal delivery. Tyrosine kinase inhibitors were highly efficient in mice, especially imatinib and genistein, and promoted transduction even of the outer retina. In rats, however, we report that they were not effective. Even with direct proteasomal inhibition in rats, the effects upon transduction were only minimal and restricted to the inner retina. Even tyrosine capsid mutant rAAVs in rats had a transduction profile similar to wtAAV. Thus, the differences between mouse and rat, in both eye size and the inner limiting membrane, compromise the efficiency of AAV vectors penetration from the vitreous into the retina, and impact the efficacy of strategies developed to enhance intravitreal retinal rAAV transduction. Further improvement of strategies, then are required.