Nutrient control by the gas evolution in methanogenesis of methanol by Methanosarcina barkeri

A practical fed-batch culture, in which consumed amounts of methanol and other nutrients were supplied in response to a direct signal of the gas production of CH 4 and CO 2, was carried out for the cell production of methanol-utilizing Methanosarcina barkeri. In this fed-batch culture system equippe...

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Published inJournal of fermentation and bioengineering Vol. 73; no. 6; pp. 481 - 485
Main Authors Nishio, Naomichi, Kakizono, Toshihide, Silveira, Roberto G., Takemoto, Susumu, Nagai, Shiro
Format Journal Article
LanguageEnglish
Published Osaka Elsevier B.V 1992
Society for Fermentation and Bioengineering
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methanosarcina barkeri
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Consumption
Methane
Archaeobacteria
Carbon dioxide
Methanosarcinaceae
Optimization
Cell density
Methanomicrobiales
Methanosarcina barkeri
Methanogenesis
Bacteria
Nutrient
Semicontinuous
Monitoring
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Summary:A practical fed-batch culture, in which consumed amounts of methanol and other nutrients were supplied in response to a direct signal of the gas production of CH 4 and CO 2, was carried out for the cell production of methanol-utilizing Methanosarcina barkeri. In this fed-batch culture system equipped with level sensors to detect the gas production, a high cell concentration of 24.4 g/ l was attained in 175-h cultivation maintaining the optimized nutrient concentrations of methanol, NH 4 +, PO 4 3−, Na +, Mg 2+, Ca 2+, Fe 2+, Ni 2+, Co 2+ and cysteine (S source) throughout the culture.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0922-338X
DOI:10.1016/0922-338X(92)90142-H