Lipase-catalyzed synthesis of sorbitol–fatty acid esters at extremely high substrate concentrations
Lipase-catalyzed synthesis of sorbitol–fatty acid esters was performed in eutectic media with extremely high substrate concentrations. Homogeneous eutectic melts of sorbitol and fatty acids of C6–C16 were prepared using an adjuvant mixture. Enhanced homogeneity of mixtures was confirmed by X-ray dif...
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Published in | Journal of biotechnology Vol. 123; no. 2; pp. 174 - 184 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
17.05.2006
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | Lipase-catalyzed synthesis of sorbitol–fatty acid esters was performed in eutectic media with extremely high substrate concentrations. Homogeneous eutectic melts of sorbitol and fatty acids of C6–C16 were prepared using an adjuvant mixture. Enhanced homogeneity of mixtures was confirmed by X-ray diffraction analysis. The substrate concentration was 3.63–6.67
M in the eutectic media, whereas in organic media the concentration was below 0.10
M. Esters were synthesized with an immobilized
Candida antarctica lipase, and optimum conditions were analyzed. Compared to reactions in organic media, the initial reaction rate of ester synthesis and the overall productivity were significantly enhanced in eutectic media while the conversion yields were similar. Based on the kinetic analysis, highly viscous eutectic media were shown to influence the initial reaction rate and the apparent activation energy resulting in diffusion limitations. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0168-1656 1873-4863 |
DOI: | 10.1016/j.jbiotec.2005.11.006 |