Optimal Culture Conditions for Constructing Durable Biografts for Repairing the Impaired Heart -Dynamic Cell Culture with Pre-seeding

• Published in: Annals of Thoracic and Cardiovascular Surgery Vol. 17; no. 5; pp. 481 - 486
• Main Authors: Yokomuro, Hiroki, Shiono, Noritsugu, Watanabe, Yoshinori, Yoshihara, Katsunori, Koyama, Nobuya, Okada, Mitsumasa
• Format: Journal Article
• Language: English
• Published: Japan The Editorial Committee of Annals of Thoracic and Cardiovascular Surgery 2011
• Subjects: Animals; Aorta - physiology; biodegradable material; Cardiac Surgical Procedures; Cell Adhesion; Cell Culture Techniques; Cell Proliferation; Cells, Cultured; durable biograft; dynamic cell culture; Heart Diseases - pathology; Heart Diseases - surgery; Motion; Muscle, Smooth, Vascular - physiology; Muscle, Smooth, Vascular - transplantation; Myocytes, Smooth Muscle - physiology; Myocytes, Smooth Muscle - transplantation; Polyesters - chemistry; pre-seeding technique; Rats; Rats, Wistar; Regeneration; Time Factors; Tissue Engineering - methods; Tissue Scaffolds
• ISSN: 1341-1098; 2186-1005
• DOI: 10.5761/atcs.oa.10.01650

Background: Tissue engineering with cell seeded biodegradable material has attracted attention as a novel means of treating the severely impaired heart. Here, we consider optimal preparation of a durable biograft using dynamic and static cultures. Methods: Vascular smooth muscle cells (VSMCs) derived from the rat aorta were seeded onto biodegradable material P (LA/CL) (poly-L-lactide-ε-caprolactone copolymer) and cultured as follows: a) Static culture (n = 11), b) dynamic culture (n = 12), c) 0 h pre-seeding (n = 12), d) 24 h pre-seeding (n = 5) and e) 1 week pre-seeding (n = 12). Dynamic culture: Cells were cultured in spinner flasks. Pre-seeding: Static cell seeding and culture before dynamic culture. Evaluation: The conditions of the P (LA/CL) in the five groups were evaluated as cell proliferation and by histological studies. Results: VSMCs proliferated both in and on the biodegradable materials. The quality of the dynamic culture cell with pre-seeding increased. Although the duration of pre-seeding exerted no significantly different effects, cell attachment and proliferation were widely scattered in the 0 h pre-seeding group, whereas cells proliferating on the front of the scaffold obstructed proliferation inside the biodegradable material in the 1 week pre-seeding group. Conclusions: Dynamic cell culture with 24 h pre-seeding is effective for constructing ideal biografts.