Alterations in membrane-associated particle distribution during antidiuretic challenge in frog urinary bladder epithelium

• Published in: Biophysical journal Vol. 16; no. 6; pp. 627 - 639
• Main Authors: Bourguet, J., Chevalier, J., Hugon, J.S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.1976
• Subjects: Animals; Cell Membrane - drug effects; Cell Membrane - ultrastructure; Cyclic AMP - pharmacology; Epithelial Cells; Epithelium - drug effects; Epithelium - ultrastructure; Freeze Etching; Freeze Fracturing; Microscopy, Electron; Oxytocin - pharmacology; Pituitary Gland, Posterior - physiology; Rana esculenta; Tissue Extracts; Urinary Bladder - drug effects; Urinary Bladder - ultrastructure
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(76)85717-7

Frog urinary bladder epithelium has been examined by freeze-fracture electron microscopy of preparations previously fixed by glutaraldehyde either at rest or during antidiuretic challenge. All the agonists tested were observed to induce membrane particle clustering in the A face of the apical plasma membrane of granular cells. This was the case for the natural hormone (hypophysical extracts) and its presumed cellular mediator, adenosine 3',5'-monophosphate. Particle clustering was observed both in the presence and in the absence of water net flow and is thus independent of these movements. Clusters were also observed during hydrosmotic challenge by hypertonic serosal media, a condition which depresses transepithelial sodium transport. No complementary patterns of these A face clusters could be found on the B face. The significance of these membrane-associated particle clusters is discussed in terms of membrane structure and function.