Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 12; no. 3; pp. 295 - 297
• Main Authors: Singh, Kuldeep, Miller, Myrna M, Kohrt, Laura J, Scherba, Gail, Garrett, Edgar F, Fredrickson, Richard L
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.09.2011; The Korean Society of Veterinary Science
• Subjects: Animals; Antibodies, Viral - analysis; Bovine Virus Diarrhea-Mucosal Disease - blood; Bovine Virus Diarrhea-Mucosal Disease - diagnosis; Bovine Virus Diarrhea-Mucosal Disease - virology; Cattle; Diarrhea Virus 1, Bovine Viral - genetics; Diarrhea Virus 1, Bovine Viral - isolation & purification; Diarrhea Virus 2, Bovine Viral - genetics; Diarrhea Virus 2, Bovine Viral - isolation & purification; Hair - virology; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - veterinary; RNA, Viral - chemistry; RNA, Viral - genetics; Short Communication; 수의학; diagnostic tests; hair; persistent infection; bovine viral diarrhea virus; RT-PCR
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2011.12.3.295

The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.