A rapid and highly specific immunofluorescence method to detect Escherichia coli O157:H7 in infected meat samples

• Published in: International journal of food microbiology Vol. 231; pp. 54 - 62
• Main Authors: Balakrishnan, Baskar, Barizuddin, Syed, Wuliji, Tumen, El-Dweik, Majed
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.08.2016
• Subjects: E. coli O157:H7; Escherichia coli; Escherichia coli O157 - isolation & purification; Fluorescent Antibody Technique; Food Microbiology; Food safety; Humans; Immunofluorescence; Magnetic bead; Meat - microbiology; Protein-A; Sensitivity and Specificity; TRITC; Magnetic bead; Protein-A; E. coli O157:H7; Food safety; Immunofluorescence; TRITC

Developing rapid and sensitive methods for the detection of pathogenic Escherichia coli O157:H7 remains a major challenge in food safety. The present study attempts to develop an immunofluorescence technique that uses Protein-A-coated, magnetic beads as the platform. The immunofluorescence technique...