Disposition in rat of [2-3H]-trans-4-hydroxy-2,3-nonenal, a product of lipid peroxidation

• Published in: Xenobiotica Vol. 26; no. 10; p. 1087
• Main Authors: de Zwart, L L, Hermanns, R C, Meerman, J H, Commandeur, J N, Vermeulen, N P
• Format: Journal Article
• Language: English
• Published: England 01.10.1996
• Subjects: Aldehydes - pharmacokinetics; Aldehydes - urine; Animals; Feces; Injections, Intraperitoneal; Lipid Peroxidation; Male; Rats; Rats, Wistar; Tissue Distribution; Tritium
• ISSN: 0049-8254
• DOI: 10.3109/00498259609167424

1. 4-Hydroxy-2,3-nonenal (HNE) is an end product of lipid peroxidation (LPO) and a well known cytotoxic aldehyde that exhibits a variety of biological effects. In this study the in vivo disposition and covalent binding of i.p. administered [2-3H]HNE was examined in the rat. 2. It was found that several metabolites of [2-3H]HNE are excreted in urine among which at least four mercapturic acids. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) appeared to be the most abundant mercapturic acid excreted in urine (3.5% of the dose) and the excretion of the other three mercapturic acids amounted to 2% of the dose. 3. Within 48 h following i.p. administration of 5 or 25 mumol/kg bodyweight [2-3H]HNE (specific activity 4 microCi/mumol) about 25% of the radioactivity was excreted in urine, whereas 18% of the radioactivity appeared in the faeces. 4. After 48 h, 7% of the radioactivity was still present in the liver and 0.2% in other organs, but this radioactivity appeared to not to be covalently bound to cellular macromolecules. It was found that only 0.13% of the radioactivity was covalently bound in the liver and even less in other organs.