Anti-Inflammatory Activity of a Medicinal Herb Extract Mixture, HM-V, on an Animal Model of DNCB-Induced Chronic Skin Inflammation

• Published in: Plants (Basel) Vol. 10; no. 8; p. 1546
• Main Authors: Park, Sungbae, Lee, Sangmin, Weon, Youngho, Kim, Taewook, Kim, Hakwon, Lee, Taehoon
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 28.07.2021; MDPI
• Subjects: Animal models; Anti-inflammatory agents; Atopic dermatitis; Biomarkers; CCL17 protein; CCL22 protein; chemokine; Chemokines; chronic skin inflammation; Communication; Contact dermatitis; Cytokines; Dermatitis; Dinitrofluorobenzene; Erythema; Gene expression; Herbal medicine; Herbs; IL-1β; Immune system; Inflammation; Inflammatory diseases; Interferon; Interleukin 6; Keratinocytes; Lymphatic system; medicinal herb extract; Medicinal plants; mRNA; Signal transduction; Skin diseases; Spleen; Tumor necrosis factor-TNF; Tumor necrosis factor-α; γ-Interferon
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants10081546

Chronic inflammatory skin diseases, such as atopic dermatitis, are caused by the accumulation of immune cells and the overproduction of chemokines, including CCL17 and CCL22, due to the activation of pro-inflammatory cytokines secreted from keratinocytes. In the present study, the inhibitory activity of HM-V on tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ)-induced pro-inflammatory cytokines was examined in human keratinocytes (HaCaTs) and 2,4-dinitrofluorobenzene (DNCB)-induced chronic skin contact dermatitis animal models. Traditional Asian medicinal herb extracts mixture (HM-V), which have been extensively used in Asian medicine, were utilized. In TNF-α/IFN-γ-induced HaCaTs, HM-V strongly inhibited mRNA and protein expression of CCL17 and CCL22 in a concentration-dependent manner. The expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 was also inhibited. Therefore, localized administration of HM-V in the DNCB-induced animal model alleviated immune cell deposition and skin inflammation. The results indicate that HM-V exerts inhibitory effects on keratinocyte production of CCL17 and CCL22. Furthermore, HM-V may be a useful anti-inflammatory agent for the prevention and treatment of inflammatory skin diseases.