Copper exposure effects on yeast mitochondrial proteome

• Published in: Journal of proteomics Vol. 74; no. 11; pp. 2522 - 2535
• Main Authors: Banci, Lucia, Bertini, Ivano, Ciofi-Baffoni, Simone, D'Alessandro, Annamaria, Jaiswal, Deepa, Marzano, Valeria, Neri, Sara, Ronci, Maurizio, Urbani, Andrea
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.10.2011
• Subjects: Cell survival; Copper; Copper - pharmacology; Data processing; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Environmental Exposure - adverse effects; Heavy metals; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; LC-MS/MS; Liquid chromatography; Mass spectroscopy; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondrial Proteins - analysis; Mitochondrial Proteins - metabolism; Models, Biological; Organelles; Oxidation-Reduction; Oxidative stress; Proteome - drug effects; Proteomics; Proteomics - methods; Reviews; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - drug effects; Saccharomyces cerevisiae Proteins - metabolism; Water Pollutants, Chemical - adverse effects; Water Pollutants, Chemical - pharmacology; Oxidative stress; LC-MS/MS; Cu; Proteomics
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2011.04.014

Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In these study we have performed an organelle targeted proteomic investigation focusing our attention on the effect of non-lethal 1 mM copper concentration on Saccharomyces cerevisiae mitochondrial proteome. Functional copper effects on yeast mitochondrial proteome were evaluated by using both 2D electrophoresis (2-DE) and liquid chromatography coupled with tandem mass spectrometry. Proteomic data have been then analyzed by different unsupervised meta-analysis approaches that highlight the impairment of mitochondrial functions and the activation of oxidative stress response. Interestingly, our data have shown that stress response generated by 1 mM copper treatment determines the activation of S. cerevisiae survival pathway. To investigate these findings we have treated yeast cells responsiveness to copper with hydrogen peroxide and observed a protective role of this metal. These results are suggestive of a copper role in the protection from oxidative stress possibly due to the activation of mechanisms involved in cellular survival and growth. [Display omitted]