Karyotype Analysis of Four Vicia Species using In Situ Hybridization with Repetitive Sequences

• Published in: Annals of botany Vol. 91; no. 7; pp. 921 - 926
• Main Authors: NAVRÁTILOVÁ, ALICE, NEUMANN, PAVEL, MACAS, JIŘÍ
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.06.2003; Oxford Publishing Limited (England)
• Subjects: Biological taxonomies; Cell Division - genetics; Cell Division - physiology; Chromosomes; Conserved Sequence - genetics; DNA, Ribosomal - genetics; Evolutionary genetics; Genetic hybridization; Genetic Markers - genetics; Genome, Plant; Genomes; in situ hybridization; In Situ Hybridization, Fluorescence; Karyotype; karyotype evolution; Karyotyping - methods; Key words:Vicia; Original; Plants; Primed In Situ Labeling; PRINS; repetitive DNA; Repetitive Sequences, Nucleic Acid - genetics; Ribosomal DNA; RNA, Ribosomal - genetics; rRNA genes; satellite DNA; Tandem Repeat Sequences - genetics; Vicia - genetics; Vicia - metabolism; Vicia sativa - genetics; Vicia sativa - metabolism
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mcg099

Mitotic chromosomes of four Vicia species (V. sativa, V. grandiflora, V. pannonica and V. narbonensis) were subjected to in situ hybridization with probes derived from conserved plant repetitive DNA sequences (18S–25S and 5S rDNA, telomeres) and genus‐specific satellite repeats (VicTR‐A and VicTR‐B). Numbers and positions of hybridization signals provided cytogenetic landmarks suitable for unambiguous identification of all chromosomes, and establishment of the karyotypes. The VicTR‐A and ‐B sequences, in particular, produced highly informative banding patterns that alone were sufficient for discrimination of all chromosomes. However, these patterns were not conserved among species and thus could not be employed for identification of homologous chromosomes. This fact, together with observed variations in positions and numbers of rDNA loci, suggests considerable divergence between karyotypes of the species studied.