Stimulus-induced oscillations in guard cell cytosolic free calcium

Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ([Ca2+]i) are transduced into different physiological responses remains to be explained. Oscillations in [Ca2+]i may...

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Published inThe Plant cell Vol. 7; no. 8; pp. 1207 - 1219
Main Authors McAinsh, M.R. (Lancaster University, Lancaster, United Kingdom.), Webb, A.A.R, Taylor, J.E, Hetherington, A.M
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.08.1995
Subjects
CALCIO
CALCIUM
CATION
CATIONES
Cell membranes
CELLULE
CELULAS
CITOPLASMA
COMMELINA
CYTOPLASME
ESTOMA
ESTRUCTURA CELULAR
Fluorescence
Guard cells
Imaging
INHIBICION
INHIBITION
ION
IONES
MANGANESE
MANGANESO
MOUVEMENT
MOVIMIENTO
Optical filters
Photometry
Plant cells
Ratios
Stomata
STOMATE
STRUCTURE CELLULAIRE
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Summary:Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ([Ca2+]i) are transduced into different physiological responses remains to be explained. Oscillations in [Ca2+]i may provide one way in which this can occur. We used photometric and imaging techniques to examine this hypothesis in guard cells of Commelina communis. External Ca2+ ([Ca2+]e), which causes an increase in [Ca2+]i, was used as a closing stimulus. The total increase in [Ca2+]i was directly related to the concentration of [Ca2+]e, both of which correlated closely with the degree of stomatal closure. Increases were oscillatory in nature, with the pattern of the oscillations dependent on the concentration of [Ca2+]e. At 0.1 mM, [Ca2+]e induced symmetrical oscillations. In contrast, 1.0 mM [Ca2+]e induced asymmetric oscillations. Oscillations were stimulus dependent and modulated by changing [Ca2+]e. Experiments using Ca2+ channel blockers and Mn2+-quenching studies suggested a role for Ca2+ influx during the oscillatory behavior without excluding the possible involvement of Ca2+ release from intracellular stores. These data suggest a mechanism for encoding the information required to distinguish between a number of different Ca2+-mobilizing stimuli in guard cells, using stimulus-specific patterns of oscillations in [Ca2+]i
Bibliography:F60
9611408
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ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.7.8.1207