Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics

Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample varia...

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Published inMetabolites Vol. 10; no. 6; p. 229
Main Authors González-Domínguez, Raúl, González-Domínguez, Álvaro, Sayago, Ana, Fernández-Recamales, Ángeles
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 03.06.2020
MDPI
Subjects
Amino acids
Anticoagulants
blood
collection
Metabolism
Metabolites
Metabolomics
Plasma
Potassium
pre-analytics
processing
Review
Thawing
Urine
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Summary:Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample variability driven by these technical issues, as well as for maintaining the metabolic integrity of biological samples to ensure that metabolomic profiles are a direct expression of the in vivo biochemical status. This review article provides an updated literature revision of the most important factors related to sample handling and pre-processing that may affect metabolomics results, particularly focusing on the most commonly investigated biofluids in metabolomics, namely blood plasma/serum and urine. Finally, we also provide some general recommendations and best practices aimed to standardize and accurately report all these pre-analytical aspects in metabolomics research.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo10060229