Chemical studies on the polysaccharides of Salicornia brachiata

• Published in: Carbohydrate polymers Vol. 112; pp. 300 - 307
• Main Authors: Sanandiya, Naresh D., Siddhanta, A.K.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 04.11.2014; Elsevier
• Subjects: Applied sciences; Biological and medical sciences; Carbohydrate Conformation; Chemical constitution; Chenopodiaceae; Chenopodiaceae - chemistry; Circular Dichroism; Exact sciences and technology; Fucose - analysis; Fundamental and applied biological sciences. Psychology; Glycosidic linkage analysis; Indian halophytes; Magnetic Resonance Spectroscopy; Monosaccharides - analysis; Natural polymers; Oxalic Acid - chemistry; Physicochemistry of polymers; Plant Extracts - chemistry; Plant physiology and development; Plant Roots - chemistry; Plant Stems - chemistry; Polysaccharides; Polysaccharides - analysis; Polysaccharides - chemistry; Salicornia brachiata; Spectroscopy, Fourier Transform Infrared; Starch and polysaccharides; Uronic acid, Arabinose, Galactose, Xylose, Glucose, Rhamnose, Fucose, Mannose, Ribose; Glycosidic linkage analysis; Polysaccharides; Salicornia brachiata; Indian halophytes; Chenopodiaceae; Uronic acid, Arabinose, Galactose, Xylose, Glucose, Rhamnose, Fucose, Mannose, Ribose; Circular dichroism spectrum; Optically active polymer; Branched polymer; Root; Molecular structure; Experimental study; Stem; Dicotyledones; Optical properties; Clycosidic linkage analysisUronic acid, Arabinose, Galactose, Xylose, Glucose, Rhamnose, Fucose, Mannose, Ribose; Angiospermae; Plant origin; Optical activity; Oside polymer; Above ground plant part; Spermatophyta; Chemical composition; Aqueous solution
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.05.072

Chemical profiling of polysaccharides of the three parts of Salicornia brachiata (tips, stem and roots) were done, which contained no toxic metal ions. Each one of the polysaccharide extract samples was unique in composition and chiroptical make up, which is of biogenetic significance. •Polysaccharides of the Indian halophyte Salicornia brachiata were chemically studied.•Different aqueous extracts of the tips, stem and roots of the plant were separately investigated.•The polysaccharides were heterogeneous and highly branched containing 5-8 natural carbohydrates.•Fucose was present in only ammonium oxalate extract of roots along with other seven carbohydrates. A group of 12 polysaccharide extracts were prepared from the tips, stem and roots of an Indian halophyte Salicornia brachiata Roxb. obtained by sequential extractions with cold water (CW), hot water (HW), aqueous ammonium oxalate (OX) and aqueous sodium hydroxide (ALK) solutions. Monosaccharide composition analysis revealed that all the polysaccharide extract samples consisted primarily of rhamnose, arabinose, mannose, galactose, glucose, whereas ribose and xylose were present only in some of the extracts. All the extracts exhibited low apparent viscosity (1.47–2.02cP) and sulphate and contained no prominent toxic metal ions. Fucose was detected only in OX extract of the roots. These polysaccharides were found to be heterogeneous and highly branched (glycoside linkage analysis, size-exclusion chromatography, 13C-NMR, FT-IR, circular dichroism and optical rotation data). Physico-chemical analyses of these polysaccharides including uronic acid, sulphate and protein contents were also carried out. This constitutes the first report on the profiling of Salicornia polysaccharides.