Inhibition of iron-oxidizing activity by bisulfite ion in Thiobacillus ferrooxidans

• Published in: Bioscience, biotechnology, and biochemistry Vol. 58; no. 11; pp. 2109 - 2110
• Main Authors: Sugio, Tsuyoshi, Iwahori, Kenji, Uemura, Satomi, Makino, Ikuko, Tano, Tatsuo
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1994; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: bacillus; Bacteriology; Biological and medical sciences; fer; ferroxidasa; ferroxidase; ferroxydase; Fundamental and applied biological sciences. Psychology; hierro; iron; Metabolism. Enzymes; Microbiology; oxidacion; oxidation; oxydation; sulfure; sulfuros; sulphides; Enzymatic activity; Disulfites; Investigation method; Enzyme; Cytochrome-c oxidase; Plasma membrane; Enzyme inhibitor; Bacteria; Oxidoreductases; Thiobacillus ferrooxidans
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.58.2109

Growth of Thiobacillus ferrooxidans on iron- and sulfur-salts media and iron oxidizing activity of this bacterium were strongly inhibited by bisulfite ion. The mechanism of inhibition by bisulfite ion of iron-oxidizing activity was studied with the plasma membrane of T. ferrooxidans AP19-3. The c-type cytochrome in the plasma membrane was reduced by ferrous ion and the cytochrome reduced by Fe 2+ was oxidized by cytochrome c oxidase in the plasma membrane. In contrast, c-type cytochrome was reduced by bisulfite ion, but it was not oxidized by cytochrome c oxidase in the membrane. Cytochrome c-oxidizing activity was also inhibited by the ion when mammalian cytochrome c was used as an electron donor, suggesting that cytochrome c oxidase, one of the component of iron oxidase, is the site of inhibition by bisulfite ion.