Isolation and Sequence of a Human Apolipoprotein CII cDNA Clone and Its Use to Isolate and Map to Human Chromosome 19 the Gene for Apolipoprotein CII

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 10; pp. 2945 - 2949
• Main Authors: Jackson, Cynthia L., Gail A. P. Bruns, Breslow, Jan L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.05.1984; National Acad Sciences
• Subjects: Adult; Amino Acid Sequence; Amino acids; Animals; Apolipoprotein C-II; Apolipoproteins - genetics; Apolipoproteins C; Base Sequence; Cell Line; Chromosome Mapping; Chromosome translocation; Chromosomes; CII region; Cloning, Molecular; Complementary DNA; Cricetinae; Cricetulus; DNA; DNA - isolation & purification; DNA Restriction Enzymes; Genes; Genetic mapping; Humans; Hybrid Cells - physiology; Hypoxanthine Phosphoribosyltransferase - genetics; Leukocytes - physiology; Lipoproteins; Liver - metabolism; Messenger RNA; Mice; Rats
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.10.2945

cDNA clones encoding human apolipoprotein CII (apo CII) were identified by screening an adult human liver cDNA library with a mixed oligonucleotide probe corresponding to all possible codons for apo CII amino acid 6-10. One clone with an ≈ 500-base-pair (bp) insert, designated pCII-711, was selected for DNA sequence analysis. This clone contained a DNA sequence that corresponded with the previously reported amino acid sequence of apo CII with only minor differences. The DNA sequence specified a polypeptide of 79 amino acids, compared to the 78 amino acids previously reported. The pCII-711 clone contains a 36-bp DNA sequence upstream from that specifying the NH2-terminal threonine which, when read in frame, specifies the amino acid sequence Leu-Val-Leu-Leu-Val-Leu-Gly-Phe-Glu-Val-Gln-Gly and may be part of an apo CII signal peptide. The pCII-711 clone also contains a 144-bp region that corresponds to the 3′untranslated region of apo CII mRNA as well as a portion of the poly(A) tail. Clone pCII-711 was used to isolate and characterize by restriction endonuclease digestion the gene for apo CII from a human genomic library. In addition, through Southern blot analysis of DNA from human-rodent somatic cell hybrids, clone pCII-711 also was used to provisionally map the gene for apo CII to human chromosome 19.