Identification of a Point Mutation in the para-Type Sodium Channel Gene from a Pyrethroid-Resistant Cattle Tick

• Published in: Biochemical and biophysical research communications Vol. 261; no. 3; pp. 558 - 561
• Main Authors: He, Haiqi, Chen, Andrew C, Davey, Ronald B, Ivie, G.Wayne, George, John E
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11.08.1999
• Subjects: acaricide; acaricide resistance; Amino Acid Sequence; Animals; Batrachotoxins - pharmacology; Boophilus; Boophilus microplus; Conserved Sequence; DNA, Complementary - chemistry; genbank/af134216; insecticide; Insecticide Resistance - genetics; Insecticides; Ixodidae; Molecular Sequence Data; Point Mutation; Polymerase Chain Reaction; Pyrethrins; resistance; Sequence Alignment; Sequence Analysis, DNA; Sodium Channels - chemistry; Sodium Channels - genetics; Ticks - genetics; acaricide; insecticide; Boophilus microplus; resistance
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1999.1076

To investigate the molecular mechanism of resistance to pyrethroids in the southern cattle tick, Boophilus microplus, we have obtained and sequenced a partial para-homologous sodium channel cDNA from susceptible and pyrethroid-resistant tick strains. A point mutation that results in an amino acid change from Phe to Ile was identified in the highly conserved domain IIIS6 of the homologous sodium channel from ticks that are highly resistant to pyrethroid acaricides. This mutation is at a location different from those reported in the same gene in pyrethroid-resistant insects.