Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 1; pp. 118 - 124
• Main Authors: Nemec, Alexandr, Musilek, Martin, Maixnerova, Martina, De Baere, Thierry, van der Reijden, Tanny J. K, Vaneechoutte, Mario, Dijkshoorn, Lenie
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2009; Society for General Microbiology
• Subjects: Acinetobacter - classification; Acinetobacter - genetics; Acinetobacter - growth & development; Acinetobacter - isolation & purification; Acinetobacter Infections - microbiology; Animals; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Culture Media; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; DNA-Directed RNA Polymerases - genetics; Environmental Microbiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hemolysis; Humans; Microbiology; Miscellaneous; Nucleic Acid Hybridization; Phenotype; Phylogeny; Polymorphism, Restriction Fragment Length; Restriction Mapping; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Systematics; Human; Taxonomy; Neisseriaceae; Bacteria; Micrococcales; Acinetobacter; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.001230-0

1 Centre of Epidemiology and Microbiology, National Institute of Public Health, robárova 48, 100 42 Prague 10, Czech Republic 2 Department of Clinical Chemistry, Microbiology and Immunology, University Hospital, Blok A, B-9000 Ghent, Belgium 3 Department of Infectious Diseases, Leiden University Medical Centre C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands Correspondence Alexandr Nemec anemec{at}szu.cz The taxonomic status of 24 haemolytic, non-glucose acidifying Acinetobacter strains that did not belong to any previously described species was investigated by means of a polyphasic approach. Using AFLP fingerprinting, amplified rDNA restriction analysis and phenotypic characterization, the strains were classified into two phenetically coherent groups (comprising 15 and 9 strains) that were distinct from each other and from all known Acinetobacter species. Confirmation that these groups formed two separate lineages within the genus Acinetobacter was obtained from comparative analysis of partial sequences of the gene encoding the β -subunit of RNA polymerase in all strains and also from 16S rRNA gene sequence analysis of representative strains. Previously published DNA–DNA reassociation data for some of the strains used also supported the species rank for both groups, for which the names Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov. are proposed. The strains of A. beijerinckii sp. nov. originated from human and animal specimens and from various environmental sources, whereas those of A. gyllenbergii sp. nov. were isolated exclusively from human clinical specimens. The phenotypic characteristics most useful for the differentiation of these species from other Acinetobacter species that comprise haemolytic strains were the inability of A. beijerinckii sp. nov. to grow on L -arginine and the ability of A. gyllenbergii sp. nov. to grow on azelate. The type strain of A. beijerinckii sp. nov. is NIPH 838 T (=LUH 4759 T =CCUG 51249 T =CCM 7266 T =58a T ) and the type strain of A. gyllenbergii sp. nov. is NIPH 2150 T (=RUH 422 T =CCUG 51248 T =CCM 7267 T =1271 T ). Abbreviations: ARDRA, amplified rDNA restriction analysis The GenBank/EMBL/DDBJ accession numbers for the rpoB partial gene sequences determined in this study are EU477105 –EU477158 and those for the 16S rRNA gene sequences of strains NIPH 838 T , LUH 6214, NIPH 2150 T , LUH 1740 and LUH 1737 are AJ626712 , AJ303013 , AJ293694 , AJ293693 and AJ293692 , respectively. Detailed data on the origins of the A. beijerinckii sp. nov. and A. gyllenbergii sp. nov. strains and a dendrogram derived from a cluster analysis of AFLP fingerprints are available as supplementary material with the online version of this paper.