Blue Light Differentially Modulates Cell Survival and Growth

Previous studies have reported that blue light (400–500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling ti...

Full description

Saved in:
Bibliographic Details
Published inJournal of dental research Vol. 83; no. 2; pp. 104 - 108
Main Authors Wataha, J.C., Lewis, J.B., Lockwood, P.E., Hsu, S., Messer, R.L., Rueggeberg, F.A., Bouillaguet, S.
Format Journal Article
LanguageEnglish
Published United States SAGE Publications 01.02.2004
SAGE PUBLICATIONS, INC
Subjects
Animals
Breast Neoplasms - pathology
Carcinoma - pathology
Carcinoma, Squamous Cell - pathology
Cell Division - radiation effects
Cell Line
Cell Line, Tumor
Cell Survival - radiation effects
Dentistry
Female
Fibroblasts - enzymology
Fibroblasts - radiation effects
Gingiva - pathology
Humans
Keratinocytes - enzymology
Keratinocytes - radiation effects
Lasers
Light
Lighting - instrumentation
Lung - pathology
Mice
Mice, Inbred BALB C
Mitochondria - radiation effects
Mouth Neoplasms - pathology
Skin - pathology
Succinate Dehydrogenase - radiation effects
fibroblasts
visible light
keratinocytes
MTT
in vitro
Online AccessGet full text

Cover

More Information
Summary:Previous studies have reported that blue light (400–500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910408300204