P19 Embryonal carcinoma cells: a new model for the study of endothelial cell differentiation

• Published in: Biotechnology letters Vol. 30; no. 7; pp. 1169 - 1175
• Main Authors: Choi, Seung-Cheol, Choi, Ji-Hyun, Shim, Wan-Joo, Lim, Do-Sun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2008; Springer; Springer Nature B.V
• Subjects: Animals; Antigens, Differentiation - biosynthesis; Applied Microbiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cancer; Cell differentiation; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cryoprotective Agents - pharmacology; Dimethyl Sulfoxide - pharmacology; Embryonal Carcinoma Stem Cells - cytology; Embryonal Carcinoma Stem Cells - metabolism; Embryos; Endothelial Cells - cytology; Endothelial Cells - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Life Sciences; Mice; Microbiology; Models, Biological; Original Research Paper; Vascular endothelial growth factor; Endothelial cell differentiation; Embryoid body; Carcinoma cells; P19 embryonal carcinoma cells; Vascular endothelial growth factor; Dimethyl sulphoxide; Endothelial cell; Vascular endothelium growth factor; Embryoid body .Endothelial cell differentiation; Carcinoma cells .Dimethyl sulphoxide; Models; Cell differentiation; Tumor cell
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-008-9677-6

P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.