Plasma miR-1273g-3p acts as a potential biomarker for early Breast Ductal Cancer diagnosis

• Published in: Anais da Academia Brasileira de Ciências Vol. 92; no. 1; p. e20181203
• Main Authors: Guo, Huan, Zeng, Xiangting, Li, Haining, Guo, Yanxiang, Wang, Tao, Guo, Hongyun, Zhu, Gongjian, Wang, Lan, Zhou, Haihong, Liu, Kedan, Chen, Xiaoyi, Wang, Haitao, Zhao, Xinke, Su, Haixiang, Li, Yingdong
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Academia Brasileira de Ciências 01.01.2020
• Subjects: biomarker; Biomarkers, Tumor - blood; breast ductal carcinoma; Breast Neoplasms - blood; Breast Neoplasms - diagnosis; Carcinoma, Intraductal, Noninfiltrating - blood; Carcinoma, Intraductal, Noninfiltrating - diagnosis; circulating miRNA; Early Detection of Cancer; Female; Humans; MicroRNAs - blood; Middle Aged; miR-1273g-3p; MULTIDISCIPLINARY SCIENCES; Neoplasm Staging; plasma; Real-Time Polymerase Chain Reaction; ROC Curve; Sensitivity and Specificity; circulating miRNA; breast ductal carcinoma; plasma; biomarker; miR-1273g-3p
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/0001-3765202020181203

Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer's disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also revealed the significantly diagnostic ability of miR-1273g-3p in patients (p=0.0414). In addition, the plasma level of miR-1273g-3p was closely related to IIIB-IIIC TNM stage. We also confirmed the higher expression level of miR-1273g-3p in breast cancer cell lines MCF-7 (4.872±0.537) than normal breast cells (Hs 578Bst). Taken together, miR-1273g-3p could represent as a potential biomarker for early breast ductal cancer diagnosis.