Modulated illumination localization microscopy-enabled sub-10 nm resolution

Optical microscopy is an essential tool for exploring the structures and activities of cells and tissues. To break the limit of resolution caused by diffraction, researchers have made continuous advances and innovations to improve the resolution of optical microscopy since the 1990s. These contribut...

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Bibliographic Details
Published inJournal of innovative optical health science Vol. 15; no. 2
Main Authors Sun, Yile, Yin, Lu, Cai, Mingxuan, Wu, Hanmeng, Hao, Xiang, Kuang, Cuifang, Liu, Xu
Format Journal Article
LanguageEnglish
Published Singapore World Scientific Publishing Company 01.03.2022
World Scientific Publishing Co. Pte., Ltd
World Scientific Publishing
Subjects
Fluorescence microscopy
Illumination
Image resolution
Light microscopy
Localization
Microscopes
Microscopy
modulated illumination
Optical microscopy
single molecule localization microscopy
Fluorescence microscopy
modulated illumination
single molecule localization microscopy
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Summary:Optical microscopy is an essential tool for exploring the structures and activities of cells and tissues. To break the limit of resolution caused by diffraction, researchers have made continuous advances and innovations to improve the resolution of optical microscopy since the 1990s. These contributions, however, still make sub-10 nm imaging an obstacle. Here, we name a series of technologies as modulated illumination localization microscopy (MILM), which makes ultra-high-resolution imaging practical. Besides, we review the recent progress since 2017 when MINFLUX was proposed and became the inspiration and foundation for the follow-up development of MILM. This review divides MILM into two types: point-scanning and wide-field. The schematics, principles and future research directions of MILM are discussed elaborately.
ISSN:1793-5458
1793-7205
DOI:10.1142/S179354582230004X