Polymorphic microsatellite markers for the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae), a vector of human African trypanosomiasis

• Published in: Molecular ecology resources Vol. 8; no. 6; pp. 1506 - 1508
• Main Authors: BROWN, J.E, KOMATSU, K.J, ABILA, P.P, ROBINSON, A.S, OKEDI, L.M.A, DYER, N, DONNELLY, M.J, SLOTMAN, M.A, CACCONE, A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.11.2008; Blackwell Publishing Ltd
• Subjects: genetic marker; genetic markers; Glossina fuscipes; microsatellite loci; sleeping sickness; trypanosomiasis; tsetse
• ISSN: 1755-0998; 1755-098X; 1755-0998
• DOI: 10.1111/j.1755-0998.2008.02328.x

Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has been severely constrained by a lack of genetic markers for mapping and population genetic studies. Here we present 10 newly developed microsatellite loci for this tsetse species. Heterozygosity levels in Moyo, an Ugandan population, averaged 0.57, with only two loci showing very low heterozygosity. Five loci carried more than six alleles. Together with five recently published microsatellite loci, this brings the number of available microsatellite loci for this species to 15. Their availability will greatly facilitate future studies on the genetics of this important human disease vector.