On-line measurement of intramolecular carbon isotope distribution of acetic acid by continuous-flow isotope ratio mass spectrometry
Molecular and intramolecular carbon isotope measurements of acetic acid present in natural environments have been performed by off‐line procedures. The off‐line method is complicated and time‐consuming and requires micromolar to millimolar amounts of sample. This limits geochemical isotopic studies,...
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Published in | Rapid communications in mass spectrometry Vol. 16; no. 11; pp. 1059 - 1064 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.01.2002
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Subjects | |
Online Access | Get full text |
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Summary: | Molecular and intramolecular carbon isotope measurements of acetic acid present in natural environments have been performed by off‐line procedures. The off‐line method is complicated and time‐consuming and requires micromolar to millimolar amounts of sample. This limits geochemical isotopic studies, especially at the intramolecular level, on acetic acid present in natural samples. Here, we examine an on‐line measurement of intramolecular carbon isotope distribution of acetic acid using continuous‐flow isotope ratio mass spectrometry (CF‐IRMS) coupled with an on‐line pyrolysis system. This is achieved by measurement of the respective carbon isotope ratios of CH4 and CO2 produced by on‐line pyrolysis of acetic acid. Results for authentic standards of pure acetic acid demonstrated the practicality of this on‐line method, although the carbon isotope ratio of the methyl group could not be determined directly. The precision of the carbon isotope measurements was 0.4‰ (1σ). The carbon isotope distribution determined by the on‐line method was identical to that determined by the conventional off‐line method within analytical error. The advantages of the on‐line method compared with the conventional off‐line method are that it is less laborious, requires less analytical time (less than one hour per sample) and, most importantly, uses smaller sample sizes (ca. 10 nanomole). An application of this on‐line method to natural geochemical samples will provide an insight into the geochemical cycle of acetic acid. Copyright © 2002 John Wiley & Sons, Ltd. |
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Bibliography: | istex:729D3470DDE2E484C2F0BAD3EF987C7262EFF3F6 ark:/67375/WNG-TFWGR52F-3 ArticleID:RCM678 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0951-4198 1097-0231 |
DOI: | 10.1002/rcm.678 |