Comparison of four molecular methods to type Salmonella Enteritidis strains

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 123; no. 5; pp. 422 - 426
• Main Authors: Campioni, Fábio, Pitondo-Silva, André, Bergamini, Alzira M.M., Falcão, Juliana P.
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01.05.2015; Wiley Subscription Services, Inc
• Subjects: Animals; Brazil - epidemiology; Chickens - microbiology; Eggs; Electrophoresis, Gel, Pulsed-Field - methods; Epidemics; ERIC-PCR; Food Microbiology; Humans; Methods; Minisatellite Repeats; MLST; MLVA; molecular epidemiology; Molecular Epidemiology - methods; Molecular Typing - methods; Multilocus Sequence Typing - methods; PFGE; Polymerase Chain Reaction - methods; Poultry; Salmonella; Salmonella Enteritidis; Salmonella enteritidis - classification; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification; Salmonella Food Poisoning - epidemiology; Salmonella Food Poisoning - microbiology; Brazil; molecular epidemiology; PFGE; MLST; ERIC-PCR; MLVA; Salmonella Enteritidis
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1111/apm.12367

This study compared the pulsed‐field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus‐PCR (ERIC‐PCR), multilocus variable‐number of tanden‐repeat analysis (MLVA), and multilocus sequence typing (MLST) methods for typing 188 Salmonella Enteritidis strains from different sources isolated over a 24‐year period in Brazil. PFGE and ERIC‐PCR were more efficient than MLVA for subtyping the strains. However, MLVA provided additional epidemiological information for those strains. In addition, MLST showed the Brazilian strains as belonging to the main clonal complex of S. Enteritidis, CC11, and provided the first report of two new STs in the S. enterica database but could not properly subtype the strains. Our results showed that the use of PFGE or ERIC‐PCR together with MLVA is suitable to efficiently subtype S. Enteritidis strains and provide important epidemiological information.