Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

• Published in: Journal of Coloproctology Vol. 35; no. 2; pp. 90 - 99
• Main Authors: Martinez, Carlos Augusto Real, Rodrigues, Murilo Rocha, Sato, Daniela Tiemi, da Silva, Camila Morais Gonçalves, Kanno, Danilo Toshio, Mendonça, Roberta Laís dos Santos, Pereira, José Aires
• Format: Journal Article
• Language: English
• Published: Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil Elsevier Editora Ltda 01.06.2015; Thieme Revinter Publicações Ltda
• Subjects: Estresse Oxidativo; Gastroenterology and Hepatology; Lipid peroxidation; Malondialdehyde; Malondialdeído; Mieloperoxidase; Myeloperoxidase; Original Article; Oxidative stress; Peroxidação dos lípidos; Ratos; Rats; Short-chain fatty acids; Sucralfate; Sucralfato; Ácidos graxos de cadeia curta (AGCC); Oxidative stress; Short-chain fatty acids; Mieloperoxidase; Malondialdeído; Lipid peroxidation; Rats; Sucralfato; Peroxidação dos lípidos; Malondialdehyde; Sucralfate; Ratos; Myeloperoxidase; Estresse Oxidativo; Ácidos graxos de cadeia curta (AGCC)
• ISSN: 2237-9363; 2317-6423
• DOI: 10.1016/j.jcol.2015.02.007

Abstract Sucralfate enemas present good results in the treatment of colitis, however the mechanism of action of the drug is not yet fully clarified. Objective To evaluate the anti-inflammatory and antioxidant effects of sucralfate enemas in diversion colitis model. Method Thirty-six Wistar rats underwent intestinal bypass by end colostomy in the descending colon and distal mucous fistula. The animals were divided into 3 experimental groups according to the daily dose of enemas received containing 0.9% SF, sucralfate enemas or sucralfate enemas 1 g/kg/day or 2 g/kg/day. Each group was divided into two subgroups according to euthanasia to be performed 2–4 weeks after derivation. The tissue grade of inflammation was assessed histologically, and neutrophil infiltration by the tissue expression of myeloperoxidase (MPO) identified by immunohistochemistry and quantified by computerized morphometry. Oxidative stress was measured by tissue levels of malondialdehyde (MDA). To compare the results the Student's t test variance was used, and also the variance by ANOVA test, establishing a level of significance of 5% ( p < 0.05) for both. Results The intervention with sucralfate enemas showed improvement in the intensity of tissue inflammation related to the concentration used and the duration of the intervention. Intervention with sucralfate enemas reduced the tissue levels of MPO, independent of concentration or time of intervention ( p < 0.01). There was a reduction of MDA levels in animals irrigated with sucralfate enemas, independent of concentration or duration of the intervention ( p < 0.01). Conclusion Enemas with sucralfate enemas reduce inflammation, neutrophil infiltration and oxidative stress in the excluded colon suggesting topical application of the substance to be a valid therapeutic option for the treatment of diversion colitis.