Impact of cigarette smoking on the middle meatus microbiome in health and chronic rhinosinusitis

• Published in: International forum of allergy & rhinology Vol. 5; no. 11; pp. 981 - 989
• Main Authors: Ramakrishnan, Vijay R., Frank, Daniel N.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.11.2015; Wiley Subscription Services, Inc
• Subjects: 16S rRNA; Adult; bacteria; Bacteria - genetics; Bacterial Infections - microbiology; Chronic Disease; endotype; Female; Humans; Male; microbiome; Microbiota; Middle Aged; Nasal Cavity - microbiology; pyrosequencing; Rhinitis - microbiology; RNA, Ribosomal, 16S - genetics; Sinuses; sinusitis; Sinusitis - microbiology; Smoking; Smoking - adverse effects; Species Specificity; sinusitis; 16S rRNA; pyrosequencing; bacteria; endotype; microbiome
• ISSN: 2042-6976; 2042-6984; 2042-6984
• DOI: 10.1002/alr.21626

Background Although cigarette smoking aggravates chronic rhinosinusitis (CRS), a detailed examination of the sinus microbiota in CRS and its clinical subtypes has yet to be performed in relation to history of smoking. Consequently, we examined associations between smoking history and sinonasal microbiome alterations in both CRS and non‐CRS populations. Methods Middle meatus swabs collected during endoscopic sinus surgery were analyzed by analysis of 16S ribosomal RNA (rRNA) sequences. Multiple analysis of variance tests were performed to determine whether microbiome composition varied with smoking history and other clinical/demographic covariates associated with CRS subtypes. Results A total 70 CRS patients and 31 control subjects were analyzed. In a univariate analysis, smoking (p = 0.04), preoperative antibiotics (p = 0.03), and purulence (p = 0.0002) were significantly associated with the genus‐level composition of the middle meatus microbiota. When included in a multivariable model, smoking was found to have significant interactions with CRS (p = 0.02), polyposis (p = 0.03), purulence (p = 0.0004), and use of saline rinses (p = 0.05). Diverse bacterial taxa differed significantly in abundance between never‐smokers and current/former smokers, as well as between different CRS subtypes. Conclusion Substantial changes in sinus bacterial colonization were observed in smokers and nonsmokers. Although the microbiota of both CRS and non‐CRS subjects were altered with smoking history, different bacterial taxa were affected by smoking in the 2 patient groups. Thus, the effects of smoking on the sinus microbiota are likely to be modified by physiological and immunological functions of the underlying sinus mucosa.