Neuronal growth‐inhibitory factor (metallothionein‐3): structure–function relationships

Neuronal growth‐inhibitory factor (GIF), also named metallothionein‐3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein‐engineered mutants...

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Published inThe FEBS journal Vol. 277; no. 14; pp. 2912 - 2920
Main Authors Ding, Zhi‐Chun, Ni, Feng‐Yun, Huang, Zhong‐Xian
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.07.2010
Subjects
Animals
Biochemistry
Cellular biology
Humans
Inhibitor drugs
metallothionein (MT)
Models, Molecular
Mutation
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - metabolism
neuronal growth‐inhibitory factor (GIF)
Protein Engineering
Simulation
Structure-Activity Relationship
structure–function relationship
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Summary:Neuronal growth‐inhibitory factor (GIF), also named metallothionein‐3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein‐engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif‐induced characteristic conformation, the solvent accessibility and dynamics of the metal–thiolate cluster, and the domain–domain interactions.
Bibliography:Department of Bioengineering, Rice University, Houston, TX 77005, USA
Immunology/Immunotherapy Program & Cancer Center, Medical College of Georgia, Augusta, GA 30909, USA
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ISSN:1742-464X
1742-4658
DOI:10.1111/j.1742-4658.2010.07716.x