Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing

• Published in: BMC research notes Vol. 10; no. 1; p. 395
• Main Authors: Wyler, Emanuel, van Heesch, Sebastiaan, Adami, Eleonora, Hubner, Norbert, Landthaler, Markus
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.08.2017; BioMed Central; BMC
• Subjects: Animals; Archives & records; BN-Lx; Genetic aspects; Genomes; Heart; Heart Ventricles - metabolism; Hypertension; Liver; Liver - metabolism; Metabolism; Methods; Myocardium - metabolism; Physiological aspects; Rat; Rats; Rats, Inbred BN; Rats, Inbred SHR; Research Note; RNA; RNA sequencing; RNA, Messenger - genetics; RNA, Ribosomal - genetics; RNA, Ribosomal - isolation & purification; Rodents; rRNA; Sequence Analysis, RNA - methods; Sequencing; SHR; Species Specificity; Splicing; Ventricle; Germany; Heart; RNA; Rat; Liver; BN-Lx; SHR; Sequencing
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-017-2716-4

The spontaneously hypertensive rat strain is a frequently used disease model. In a previous study, we measured translational efficiency from this strain and BN-Lx animals. Here, we describe long RNA sequencing reads from ribosomal RNA depleted samples from the same animals. This data can be used to investigate splicing-related events. RNA was extracted from rat liver and heart left ventricle from BN-Lx and SHR/Ola rats in biological replicates. Ribosomal RNA was removed and the samples subjected to directional high-throughput RNA-sequencing. Read and alignment statistics indicate high quality of the data. The raw sequencing reads are freely available on the NCBI short read archive and can be used for further research on tissue and strain differences, or analysed together with other published high-throughput data from the same animals.