Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

• Published in: Journal of integrative plant biology Vol. 53; no. 1; pp. 63 - 73
• Main Authors: Moe, Kyaw Thu, Chung, Jong-Wook, Cho, Young-Il, Moon, Jung-Kyung, Ku, Ja-Hwan, Jung, Jin-Kyo, Lee, Jungran, Park, Yong-Jin
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 2011; The Rural Development AdministrationRDA,Suwon 441-100,Republic of Korea%The Rural Development AdministrationRDA,Suwon 441-100,Republic of Korea%Department of Plant Resources,College of Industrial Sciences,Kongju National University,Yesan 340-702,Republic of Korea; Legume Bio-Resource Center of Green Manure(LBRCGM),Kongju National University，Yesan 340-702,Republic of Korea; Department of Plant Resources,College of Industrial Sciences,Kongju National University,Yesan 340-702,Republic of Korea%Department of Plant Resources,College of Industrial Sciences,Kongju National University,Yesan 340-702,Republic of Korea
• Subjects: Fabaceae - genetics; Gene Expression Profiling; Minisatellite Repeats; Polymorphism, Single Nucleotide; Sequence Analysis, DNA
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2010.01012.x

Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs (≥500 bp) with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat (SSR) motifs was identified in Jangan (1 630) compared with that of Sunhwa (1 334). A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms (SNPs) and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was ∼860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.