Effects of maturation and aging on the pressure-bearing region of the plantaris longus tendon of the bullfrog (Lithobates catesbeianus)

• Published in: Microscopy research and technique Vol. 77; no. 10; pp. 797 - 805
• Main Authors: Zorel, Valdenilson José, Morais, Jymenez De, de Aro, Andrea Aparecida, Gomes, Laurecir, Esquisatto, Marcelo Augusto Marretto
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.10.2014; Wiley Subscription Services, Inc
• Subjects: Age; Aging; Aging - physiology; Alterations; Animals; collagen fibers; Collagens; Compressing; extracellular matrix; Fibers; glycosaminoglycans; Glycosaminoglycans - analysis; Hydroxyproline - analysis; Maturation; Metamorphosis; Microscopy; Microscopy, Electron, Transmission; Rana catesbeiana - anatomy & histology; Rana catesbeiana - physiology; Swelling; Tendons; Tendons - chemistry; Tendons - physiology; Tendons - ultrastructure; Weight-Bearing - physiology; glycosaminoglycans; collagen fibers; aging; extracellular matrix
• ISSN: 1059-910X; 1097-0029; 1097-0029
• DOI: 10.1002/jemt.22402

ABSTRACT The plantaris longus tendon (PLT) in bullfrog develops a fibrocartilage‐like tissue in the area that is functionally subject to compressive forces. The aim of this study was to analyze the modifications of the pressure‐bearing region in bullfrog PLT with different ages (7, 180, and 1,080 days after the end of metamorphosis) using histomorphometric, ultrastructural and biochemical methods. Weak basophilia and cells with a fibroblastic phenotype were observed in the compression region at 7 days of age. On the other hand, a large area of intense tissue basophilia associated with a chondroblast‐like cell population was noted at the other ages. Collagen fibers exhibited a three‐dimensional network‐like arrangement at all ages. The number of connective tissue cells increased between 7 and 180 days of age and was reduced in older animals. The 180‐day‐old animals presented a well‐developed pericellular matrix rich in proteoglycans. The mean diameter of collagen fibrils increased from 7 to 180 days and was the same at 1,080 days. Glycosaminoglycan content was higher in 7‐day‐old animals. A higher amount of hydroxyproline was observed at 180 and 1,080 days. The swelling test showed a significant increase of wet weight in 7‐day‐old animals. In conclusion, the alterations that occur in the pressure‐bearing of bullfrog PLT are the result of physiological alterations of the animal with the maturation and aging. Microsc. Res. Tech. 77:797–805, 2014. © 2014 Wiley Periodicals, Inc.