LL-37 in periodontal health and disease and its susceptibility to degradation by proteinases present in gingival crevicular fluid

Aim To determine the levels of LL‐37 in and its susceptibility to degradation by components of gingival crevicular fluid (GCF) in periodontal health and disease. Materials and Methods Levels of LL‐37 in GCF from periodontitis patients and periodontally healthy subjects were determined by ELISA. In a...

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Published inJournal of clinical periodontology Vol. 40; no. 10; pp. 933 - 941
Main Authors McCrudden, Maelíosa T. C., Orr, David F., Yu, Yang, Coulter, Wilson A., Manning, Gwen, Irwin, Chris R., Lundy, Fionnuala T.
Format Journal Article
LanguageEnglish
Published United States Blackwell Publishing Ltd 01.10.2013
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Summary:Aim To determine the levels of LL‐37 in and its susceptibility to degradation by components of gingival crevicular fluid (GCF) in periodontal health and disease. Materials and Methods Levels of LL‐37 in GCF from periodontitis patients and periodontally healthy subjects were determined by ELISA. In addition, degradation of synthetic/exogenous LL‐37 by components of GCF in the presence and absence of inhibitors was determined by matrix‐assisted laser desorption/ionization time of flight mass spectrometry. Results The concentration of native LL‐37 in GCF from Porphyromonas gingivalis positive (Pg+) and P. gingivalis negative (Pg‐) sites in periodontitis patients was significantly higher than in GCF from healthy subjects. When synthetic LL‐37 was added to healthy GCF, the peptide was not degraded. Conversely, GCF from Pg+ sites rapidly degraded synthetic LL‐37 which was prevented in the presence of Arg− and Lys− gingipain inhibitors. Synthetic LL‐37 was degraded more slowly by GCF from Pg− sites. Conclusions LL‐37 is detectable in GCF in periodontal health and disease. The rapid degradation of synthetic LL‐37 in periodontitis GCF, particularly in Pg+ sites, limits its role as a potential therapeutic in the gingival crevice. These results highlight the need to design stable peptide mimetics of LL‐37 as future therapeutics in periodontitis.
Bibliography:ArticleID:JCPE12141
ark:/67375/WNG-DD94L95K-L
istex:0D8A34681E3183AA341A178C6BD8CBB3548F34DE
HSC Public Health Agency Research
The study was supported by the HSC Public Health Agency Research & Development office. The authors declare that there are no conflicts of interest in this study.
Conflict of interest and sources of funding statement
ISSN:0303-6979
1600-051X
DOI:10.1111/jcpe.12141